ID   NF1; HTS; version 1.
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EN   CTF/NF1
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DT   09-Aug-2005
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DE   HTP SELEX for transcription factor CTF/NF1, 4 cycles
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FN   transcription factor CTF/NF1
FC   A2
FS   recombinant protein; vaccinia system
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RN   [1]
RX   PUBMED; 12101405
RA   Roulet E, Busso S, Camargo AA, Simpson AJ, Mermod N, Bucher P.
RT   High-throughput SELEX SAGE method for quantitative modeling of 
RT   transcription-factor binding sites.
RL   Nat Biotechnol. 2002 Aug;20(8):831-5.
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EX   HTPSELEX
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NS   input; 78 bp
SQ   tccatctctt ctgtatgtcg agatctannn nnnnnnnnnn nnnnnnnnnn nntagatctc
SQ   ctaaccgact ccgttaatt
NS   vector left; 62 bp;
SQ   ggccgccagt gtgatggata tctgcagaat tccagcacac tggcggccgt tactagtgga
NS   tag unit; 33 bp;
SQ   tctannnnnn nnnnnnnnnn nnnnnnnnnt aga
NS   vector right; 62 bp;
SQ   tccgagctcg gtaccaagct tgatgcatag cttgagtatt ctatagtgtc acctaaatag
XX
SX   Cycle 0; R25_0; 467 traces; 425 clones; 854 hq-tags
SX   Cycle 1; NF1_1; 479 traces; 402 clones; 955 hq-tags
SX   Cycle 2; NF1_2; 467 traces; 367 clones; 1203 hq-tags
SX   Cycle 3; NF1_3; 1924 traces; 1425 clones; 5579 hq-tags
SX   Cycle 4; NF1_4; 315 traces; 253 clones; 309 hq-tags
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XR   gene A; HGNC; 7784; NF1A.
XR   protein A; Uniprot/Swissprot; Q12857[1 ..399]; NF1A_HUMAN
XR   factor A; TRANSFAC; T00094; NF1/CTF
XR   input; HTPSELEX:R25
XR   restriction endonuclease; REBASE:261; BglII (5' A|GATCT 3' TCTAG|A).
XR   sequencing vector; pZERO-2T;EMBL:Y10545; ECY10545
XX
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