Oh14, AUXIN RESPONSE FACTOR6 (ARF6) ChIP-seq

Description

ChIP-seq analysis of the ARF6 transcription factor. ARFs are transcription factors that bind the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs). Transgenic plants expressiong ARF6-Myc driven by native ARF6 promoter (ARF6p::ARF6-Myc) are grown in the dark for 5 days. The etiolated seedlings are then used as input for chromatin extraction with an anti-myc antibody.

Source

Data available at: GEO

Input file formats : SRA

Samples

From Arabidopsis at10 (araTha1) Assembly

Filename Description Feature GEO-ID
1 GSM1252254.sga ARF6 ARF6 GSM1252254
2 GSM1252255.sga GFP GFP GSM1252255

Technical Notes

BAM file was converted into SGA using BedTools and ChIP-Seq tools.

References

  1. Oh E, Zhu JY, Bai MY, Arenhart RA et al.

    Cell elongation is regulated through a central circuit of interacting transcription factors in the Arabidopsis hypocotyl. Elife 2014 May 27;3. 24867218