Do 2012, Mutant p53 and IgG in MDAH087 fibroblast cell line

Description

Analysis of p53 and IgG in the Li-Fraumeni cell line MDAH087, which expresses only the R248W mtp53 protein (Bischoff et al. 1990).

Source

Samples

From Human Mar. 2006 (GRCh36/hg18) Assembly.

ChIP-seq data:

Filename Description Feature GEO-ID
1 GSM900167_MDAH087_IgG.sga MDAH087 IgG IgG GSM900167
2 GSM900168_MDAH087_p53.sga MDAH087 p53 p53 GSM900168

Technical Notes

FASTQ files were extracted from SRA files using fastq-dump (SRA toolkit v2.5.0) and mapped to hg18 genome using Bowtie v0.12.8. SAM files were then converted into bam using samtools v0.1.14 and to bed using bamToBed v2.12.0 (bedtools). SGA conversion was carried out using bed2sga.pl (ChIP-seq).

References

Last update: 4 Jan 2016