Tsai 2010, TRIM24, estrogen receptor and H3K4me2 in estrogen-treated and -untreated MCF7 cells
ChIP-seq against ER, TRIM24 and H3K4me2 in estrogen treated and untreated MCF7 cells.
Includes total genomic DNA sample as control.
- Raw data was downloaded from GEO series:
- Input file format: SRA
From Human Mar. 2006 (GRCh36/hg18) Assembly.
FASTQ files were extracted from SRA files using fastq-dump
v2.5.0) and mapped to the genome
files were then converted into bam
and to bed using bamToBed v2.12.0
conversion was carried out using bed2sga.pl
Tsai WW, Wang Z, Yiu TT, Akdemir KC, Xia W, Winter S, Tsai CY, Shi X, Schwarzer D, Plunkett W, Aronow B, Gozani O, Fischle W, Hung MC, Patel DJ, Barton MC.
TRIM24 links a non-canonical histone signature to breast cancer.
Nature. 2010 Dec 16;468(7326):927-32. doi: 10.1038/nature09542.
Chromatin enrichment of TRIM24, estrogen receptor and H3K4me2 in estrogen-treated and -untreated MCF7 cells