Wu 2012, Tcf7 and Runx1 ChIP-seq in multipotential hematopoietic precursor cells, GSE31221.

Description

Tcf7 (also called Tcf1) and Runx1 ChIP-seq in multipotential hematopoietic precursor (EML for Erythroid, Myeloid, and Lymphocytic) cells derived from mouse bone morrow. EML cells were transduced with a retrovirus expressing a dominant negative retinoic acid receptor and cultured in presence of stem cell factor (SCF). CD34+ cells were sorted using MACS followed by FACS and subjected to ChIP-seq.

Source

Samples

From M. musculus (July 2007 NCBI37/mm9).

ChIP-seq data:

Filename Description Feature GEO-ID
1 GSM773994.sga EML|Tcf7 Tcf7 -
2 GSM773995.sga EML|Runx1|rep1 Runx1 -
3 GSM773996.sga EML|Runx1|rep2 Runx1 -
4 GSM773997.sga EML|Input_Tcf7 Input -
5 GSM773998.sga EML|Input_Runx1 Input -

ChIP-seq peaks files:

Filename Description Feature GEO-ID
1 Runx1_peaks.sga EML|Runx1 Runx1_P -
2 Tcf7_peaks.sga EML|Tcf7 Tcf7_P -

Technical Notes

bam read files already mapped to mm9 were downloaded and converted to bed using bamToBed v2.12.0 (bedtools). sga conversion was carried out using bed2sga.pl (ChIP-Seq v. 1.5.2). bed peak files were downloaded and converted to sga using bed2sga.pl as well.

References

Last update: 24 Jan 2017