Wu 2012, Tcf7 and Runx1 ChIP-seq in multipotential hematopoietic precursor cells, GSE31221.
Tcf7 (also called Tcf1) and Runx1 ChIP-seq in multipotential hematopoietic
precursor (EML for Erythroid, Myeloid, and Lymphocytic) cells derived from
mouse bone morrow.
EML cells were transduced with a retrovirus expressing a dominant negative
retinoic acid receptor and cultured in presence of stem cell factor (SCF).
CD34+ cells were sorted using MACS followed by FACS and subjected to ChIP-seq.
- Raw data was downloaded from: GEO
- Input file format: bam for reads, bed for peaks
- Download date: 23-1-2017
From M. musculus (July 2007 NCBI37/mm9).
ChIP-seq peaks files:
bam read files already mapped to mm9 were downloaded and converted to bed using bamToBed v2.12.0
conversion was carried out using bed2sga.pl (ChIP-Seq v. 1.5.2).
bed peak files were downloaded and converted to sga using bed2sga.pl as well.
Wu JQ, Seay M, Schulz VP, Hariharan M, Tuck D, Lian J, Du J, Shi M, Ye Z, Gerstein M, Snyder MP, Weissman S
Tcf7 is an important regulator of the switch of self-renewal and differentiation in a multipotential hematopoietic cell line.
PLoS Genet. 2012;8(3):e1002565. doi: 10.1371/journal.pgen.1002565. Epub 2012 Mar 8.
TCF7 is a key regulator of the switch of self-renewal and differentiation in a multipotential hematopoietic cell line