Lien 2014, transcriptional goverance of hair follicle stem cells by canonical Wnt regulators.

Description

Wnt regulators (TCF3, TCF4 and pan-TLE) were analyzed in hair follicle stem cells (HFSC) after FACS sorting.

Source

Samples

From M. musculus (July 2007 NCBI37/mm9).

ChIP-seq data:

Filename Description Feature GEO-ID
1 GSM1187391_qHFSC_input.sga qHFSC cells|input input GSM1187391
2 GSM1187392_qHFSC_TCF3_ChIPSeq.sga qHFSC cells|TCF3 TCF3 GSM1187392
3 GSM1187393_qHFSC_TCF4_ChIPSeq.sga qHFSC cells|TCF4 TCF4 GSM1187393
4 GSM1187394_qHFSC_TLE_ChIPSeq.sga qHFSC cells|TLE TLE GSM1187394

Technical Notes

FASTQ files were extracted from SRA files using fastq-dump (SRA toolkit v2.5.0) and mapped to the genome using Bowtie v0.12.8. SAM files were then converted into bam using samtools v0.1.14 and to bed using bamToBed v2.12.0 (bedtools). SGA conversion was carried out using bed2sga.pl (ChIP-Seq v. 1.5.3).

References

Last update: 13 Oct 2017