Wu 2012, Tcf7 and Runx1 ChIP-seq in multipotential hematopoietic precursor cells, GSE31221.

Tcf7 (also called Tcf1) and Runx1 ChIP-seq in multipotential hematopoietic precursor (EML for Erythroid, Myeloid, and Lymphocytic) cells derived from mouse bone morrow. EML cells were transduced with a retrovirus expressing a dominant negative retinoic acid receptor and cultured in presence of stem cell factor (SCF). CD34+ cells were sorted using MACS followed by FACS and subjected to ChIP-seq.

• Raw data was downloaded from: GEO
• Input file format: bam for reads, bed for peaks
• Download date: 23-1-2017

From M. musculus (July 2007 NCBI37/mm9).

ChIP-seq data:

	Filename	Description	Feature	GEO-ID
1	GSM773994.sga	EML|Tcf7	Tcf7	-
2	GSM773995.sga	EML|Runx1|rep1	Runx1	-
3	GSM773996.sga	EML|Runx1|rep2	Runx1	-
4	GSM773997.sga	EML|Input_Tcf7	Input	-
5	GSM773998.sga	EML|Input_Runx1	Input	-

ChIP-seq peaks files:

	Filename	Description	Feature	GEO-ID
1	Runx1_peaks.sga	EML|Runx1	Runx1_P	-
2	Tcf7_peaks.sga	EML|Tcf7	Tcf7_P	-

bam read files already mapped to mm9 were downloaded and converted to bed using bamToBed v2.12.0 (bedtools). sga conversion was carried out using bed2sga.pl (ChIP-Seq v. 1.5.2). bed peak files were downloaded and converted to sga using bed2sga.pl as well.

• Wu JQ, Seay M, Schulz VP, Hariharan M, Tuck D, Lian J, Du J, Shi M, Ye Z, Gerstein M, Snyder MP, Weissman S
  Tcf7 is an important regulator of the switch of self-renewal and differentiation in a multipotential hematopoietic cell line. PLoS Genet. 2012;8(3):e1002565. doi: 10.1371/journal.pgen.1002565. Epub 2012 Mar 8. 22412390
• GEO series GSE31221: TCF7 is a key regulator of the switch of self-renewal and differentiation in a multipotential hematopoietic cell line