Young 2011, ChIP-seq analysis of H3K27me3 and PolII in 2 cell lines.
ChIP-seq against H3K27me3 and, RNApol-II in mouse embryonic stem (ES) cells and haemopoietic cell line (G1ME).
As a control, whole cell extract (WCE/input) was also sequenced for both cell types without the inclusion of an antibody.
- Raw data was downloaded from GEO series: GSE27967
- Input file format: BAM
From M. musculus (July 2007 NCBI37/mm9).
BAM files were converted into BED using bamToBed v2.12.0 (bedtools
SGA conversion was carried out using bed2sga.pl (ChIP-Seq
Young MD, Willson TA, Wakefield MJ, Trounson E, Hilton DJ, Blewitt ME, Oshlack A, Majewski IJ
ChIP-seq analysis reveals distinct H3K27me3 profiles that correlate with transcriptional activity.
Nucleic Acids Res. 2011 Sep 1;39(17):7415-27. doi: 10.1093/nar/gkr416. Epub 2011 Jun 7.
ChIP-seq analysis reveals distinct H3K27me3 profiles associated with gene regulation [ChIP-seq]