ZFN

Genome-wide tag scanner for nuclease off-sites


Target Databases
Genomes:

Search ZFN Sites
Please Enter Left ZFN half-site 5' to 3' (min 6 nt - max 60 nt)

Please Enter Right ZFN half-site 5' to 3' (min 6 nt - max 60 nt)

Please Enter Allowed Spacing
     (one or two values separated by a comma)   
Allow Left and Right Protein Homo-Dimerization   
Number of Mismatches per half-site (max 2)     

Background: Based on the Tagger software, this application is intended to search genomes for specific target sites and off-target sites, such as for pairs of zinc finger proteins (ZFPs), zinc finger nucleases (ZFNs) and modified homing endonucleases. This can either define a zinc finger nucleases (hetero-dimer) or allow for homo-dimers depending on the status of the checkbox in the input form.

Reference:
Provisional PDF: BMC Bioinformatics
ZFN-Site searches genomes for zinc finger nuclease target sites and off-target sites
Thomas J Cradick, Giovanna Ambrosini, Christian Iseli, Philipp Bucher, Anton P McCaffrey
BMC Bioinformatics 2011, 12:152 (13 May 2011)

Last update 15 June 2012